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When marine molluscs such as mussels are exposed to contaminant chemicals, the lysosomes in the digestive gland epithelial cells show fairly rapid and characteristic pathological alterations (Lowe, 1988; Moore, 1988) These include swelling of the digestive cell lysosomes, accumulation of unsaturated neutral lipid in the lysosomes, increased fragility of the lysosomal membrane, and excessive build-up of lipofuscin in the lysosomal compartment These changes are accompanied by atrophy of the digestive epithelium, apparently involving augmented autophagic processes, although there is also evidence of increased cell deletion (probably analogous to apoptosis in mammals) and the relationship between the two processes, if any, is unclear (Lowe, 1988; Pipe & Moore, 1986) For example, it is not known whether the autophagic-type changes predispose the cells to deletion.

The GTS request command is one of the commands used to manage GTSs It is used when a DEV requests GTSs to allocate to it This command is also used by a DEV to request the deallocation of GTSs or allocation acknowledgments This command is used by DEVs that have a valid address The GTS request command frame contains the MHR eld, command frame ID eld, GTS control eld, and GTS characteristics eld MHR The MHR contains the destination addressing mode sub eld, source addressing mode sub eld, security sub eld, frame pending sub eld, acknowledgment request sub eld, source PAN ID eld, and source address eld The elds and sub elds all work similarly to the other command frame types GTS Control Field The GTS control eld is the third eld of the GTS request command frame.

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This eld is 8 bits in length and contains the request type sub eld, reserved sub eld, and GTS ID sub eld The request type sub eld contains the speci c type of GTS request that is being transmitted This 2-bit sub eld uses the values 00 to represent a GTS allocation, 01 to represent a GTS deallocation, 10 to represent a GTS con rmation, and 11 for the application programmer The last 3 bits of the GTS control eld is the GTS ID sub eld This speci es the ID of the GTS to which the command is referring Remember, there can only be to seven GTSs per frame CFP GTS Characteristics Field The GTS characteristics eld is composed of the GTS length eld, reserved eld, and GTS direction eld The information contained within the frame is the number of superframe slots being used for GTSs.

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Linked biochemical and cytochemical investigations have demonstrated that increased fragility of the lysosomes, induced by phenanthrene, corresponds directly with increased catabolism of cytosolic proteins (Moore & Viarengo, 1987) Experimental studies have clearly demonstrated that the lysosomal alterations described above can be induced by single toxicants such as copper and polycyclic aromatic hydrocarbons (Moore et al, 1984) At rst sight this nding is perhaps surprising given that many thousands of individual chemicals are often present in a contaminated situation However, it would appear that the pattern of lysosomal response observed is essentially very generalised and can be induced by non-chemical stressors such as hypoxia, hypothermia, osmotic shock and dietary depletion (Moore, 1985) Thus it would appear that many adverse conditions are capable of inducing autophagic-type changes This non-speci city of the lysosomal reactions is therefore of value as a general indicator of deterioration in the health of the animal.

The GTS characteristic format also gives the direction of the data frame transmissions by DEVs 1357 GTS Allocation Command.

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